Extended Data Fig. 5: ENO1 and YAP1 activate AA metabolism in a PLCB1- and HPGD-dependent manner.
From: ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
a, AA and PGE2 contents were measured in PLC cells expressing ENO1 or YAP1 by ELISA (n = 3 independent assays). b, c, Immunoblotting and qPCR analysis of HPGD expression in PLC cells expressing shRNAs targeting HPGD (b), followed by the detection of PGE2 content and cell growth status (c) (n = 3 independent assays). Actin served as a negative control. d, Protein levels of ENO1 or YAP1, and HPGD (lower panel), and PGE2 content (upper panel) were measured in ENO1 or YAP1-overexpressing PLC cells expressing HPGD (n = 3 independent assays). Actin served as a negative control. e, AA content was measured in ENO1- or YAP1-overexpressing Hep3B cells infected with shRNA viruses targeting PLCB1 (n = 3 independent assays). f, Protein levels of HPGD were measured by immunoblotting in endogenous ENO1-knockdown PLC cells expressing wild-type ENO1, ENO1S40A, and ENO1D245R. AA and PGE2 contents in these cell lines were measured by ELISA (n = 3 independent assays). Actin served as a negative control. Immunoblots are representative of three independent experiments. Error bars denote the mean ± s.e.m. (a-f) or mean ± s.d. (c, lower panel). Statistical analyses were performed by two-tailed Student’s t-test (a-f), or two-way ANOVA (c, lower panel).
