Extended Data Fig. 7: Characterization of NVS-STG2 in human PBMC and human STING KI mice BMDM.
From: Activation of human STING by a molecular glue-like compound
a, NVS-STG2 induces high level of IFN-b in all major human PBMC haplotypes (WT, REF and HAQ), in comparable level to endogenous ligand cGAMP (mean fold changes over untreated sample +/− SEM, n = 3 independent samples for each condition). b, Diagram of human STING CRISPR KI. c, Bone marrow derived macrophages (BMDM) from hSTING knock-in (KI) mice (mSTINGwt/wt, mSTINGindel/indel, hSTING+/indel, hSTING+/+) were stimulated with cGAMP (green) or NVS-STG2(red) and IP10 levels (n = 1 for each condition) were measured. cGAMP induces IP10 in both mouse and human STING mice BMDM, while NVS-STG2 only induces IP10 in mice with hSTING KI alleles.
