Extended Data Fig. 6: A PYR1*AZIN-based circuit drives RUBY gene expression in Arabidopsis in response to azinphos-ethyl.
From: An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity
Shown are images collected using two independent, single-insert homozygous transgenic lines with a GAL4-based PYR1*AZIN/HAB1* circuit driving expression of the betalain pigment marker RUBY. Foliar applications of 0, 10, 25, 50, 100, and 250 nM azinphos-ethyl to eight plants of Col-0, line #3, and line #21 were imaged 48 hours post-treatment. All solutions contain 0.05% DMSO (carrier solvent) and 0.02% Silwet (a surfactant added with agrochemicals to improve chemical uptake into inner tissues). The images show one representative seedling per treatment.
