Extended Data Fig. 3: Muscle cell-type specific mitochondrial proteome mapping using ‘floxed’ LSL-MAX-Tg results (Myf5-Cre; LSL-MAX-Tg).
From: Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis
(a) Scheme for muscle cell-specific mitochondrial matrix proteome mapping using LoxP-Stop-LoxP-MAX-Tg (LSL-MAX-Tg) mouse crossed with Myf5-Cre driver mouse. (b) Western blotting of MTS-V5-APEX2 (expected processed molecular weight: 28 kDa). Representative images from three independent experiments. (c) Streptavidin (SA)-HRP western blotting of biotinylated proteins. Representative images from three independent experiments are shown. (d) Venn diagram of identified mitochondrial matrix proteins from the tibialis anterior, quadriceps, and soleus tissues from Myf5-Cre; LSL-MAX-Tg mice. (see Supplementary Data 4 for detailed information) (e) Volcano plot of the DBP-labeled proteome labeled by MTS-APEX2 in quadriceps (left) vs. soleus (right) from Myf5-Cre; LSL-MAX-Tg mice. Analysis of statistically significant fold change revealed significantly different proteins between the quadriceps proteome and soleus proteome of Myf5-Cre; LSL-MAX-Tg mice. (f) Normalized mass intensities of asymmetrically DBP-labeled mitochondrial matrix proteins by MTS-APEX2 in the quadriceps and soleus muscle of Myf5-Cre; LSL-MAX-Tg mice.
