Extended Data Fig. 3: Effects of Ebio1 on the activity of single KCNQ2/3, KCNQ4 and KCNQ5 channels.
From: A small-molecule activation mechanism that directly opens the KCNQ2 channel
a, Representative single-channel recordings from inside-out patches of KCNQ2/3, KCNQ4 and KCNQ5 at +50 mV in the absence and presence of 10 μM Ebio1 (left panel). The corresponding all-point amplitude histograms for the sweeps were fitted by Gaussian distributions (solid line in red) (right panel). b, Histogram showing the PO of KCNQ2/3, KCNQ4 and KCNQ5 at +50 mV in the absence and presence of 10 μM Ebio1. Statistical analysis: two-tailed t-test. n.s., not significant. c, Single-channel conductance of KCNQ2/3, KCNQ4 and KCNQ5 was fitted from two peak values in (a). Statistical analysis: two-tailed t-test. n.s., not significant. n = 4, 4, 3, 3, 3 and 3 (b), n = 4, 4, 3, 3, 3 and 3 (c) biological replicates. Data are presented as the means ± s.e.m.
