Extended Data Fig. 6: OligoD insertion into H1 HA.
a, Size-exclusion purification and gel electrophoresis of wild-type or oligoD-modified H1 HA. Gel was stained with Coomassie brilliant blue. b, SEC-MALS analysis of H1 HA and H1 HA-12D on a Superdex 200 column. UV and LS indicate absorbance at 280 nm and light scattering signals, respectively. c, Thermal melting profiles and Tm of wild-type and oligoD-modified H1 HA. Data are presented as mean ± s.d. (n = 3 samples per group). d, Serum binding titers to different group 1 HAs (H1 CA/09 – A/California/07/2009, H2 JP/57 – A/Japan/305/1957, H5 VT/04 – A/Vietnam/1203/2004) (n = 10 mice per group). Each circle represents a single mouse (n = 10 mice per group). Dashed lines indicate the limit of quantification. Data are presented as the geometric mean ± s.d. of the log10-transformed values. Comparison of two groups was performed using the two-tailed Mann–Whitney U-test. P values of 0.05 or less were considered significant.
