Extended Data Fig. 3: Structure determination of RnKir4.1PIP2.
From: Pharmacological inhibition of Kir4.1 evokes rapid-onset antidepressant responses
a, Size-exclusion chromatography of RnKir4.1PIP2 on superose 6 (GE Healthcare). b, SDS-PAGE analysis of the RnKir4.1PIP2 sample (n = 3 independent experiments). c, Representative cryo-EM micrograph of RnKir4.1PIP2 (n = 2915 micrographs). d, Flowchart of image processing for RnKir4.1PIP2 particles. e, Angular distribution plot of particles included in the final C4-symmetric 3D reconstruction of RnKir4.1PIP2. f, The Gold standard Fourier Shell Correlation (FSC) curves of the final 3D reconstructions of RnKir4.1PIP2. g, The 3D reconstruction of RnKir4.1PIP2 colored by local resolution in Å. h, The FSC curves for cross-validation between the map and the model of RnKir4.1PIP2. i, Sample maps at 2 transmembrane helices of RnKir4.1PIP2 at the contour level of 5 σ. j, No density map in the central cavity of RnKir4.1PIP2 at the level of 0.01 in UCSF chimera. k, Density of PIP2 and its surrounding residues in RnKir4.1PIP2 at the contour level of 5 σ.
