Extended Data Fig. 8: Tertiary structure of Clivia_III-NBSI. | Nature Chemical Biology

Extended Data Fig. 8: Tertiary structure of Clivia_III-NBSI.

From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift

Extended Data Fig. 8

(a) The tertiary structure of Clivia_III-NBSI was shown schematically. The three NBSI binding modules were shown in blue (CM1), cyan (CM2) and magenta (CM3) from the south to north respectively. (b) Continuous stacking helical architecture was formed by the pairing residues and three bound NBSI molecules. (c) The folding of the connected stem between CM2 and CM3. A42 and A41 form a-minor interaction with the connected stem. (d) A-minor base triple interaction in module CM3 is shown and all the involved residues are represented in stick. (e-f) Two NBSI binding pockets CM2 and CM3 are shown in stick respectively. Each of them folds independently with similar conformations in Clivia_III-NBSI structure.

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