Extended Data Fig. 9: Gel-filtration analysis and in vitro fluorescence assay of multivalent Clivia fluorogenic aptamer.
From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift
(a) The solution state of multivalent Clivia fluorogenic aptamer containing varying number of NBSI binding modules from 1 to 6 were tested by size-exclusion experiment with SuperoseTM Increase 10/300 GL column. The running buffer contains 50 mM HEPES, pH 6.8, 50 mM NaCl and 5 mM MgCl2. All the RNA molecules exist homogenously as monomers in solution. (b) In vitro fluorescence assay of multivalent Clivia fluorogenic aptamer reveal increased fluorescence intensity with an increasing number of NBSI binding modules, all in the presence of the same concentration of NBSI. The activated fluorescence of NBSI by multivalent Clivia fluorogenic aptamer from three independently repeated experiments are normalized for comparison with the single WT Clivia aptamer. Data represent the mean ± s.d. from three replicates.
