Extended Data Fig. 3: Fluorescence assay of U7 mutants and metal dependence test of Clivia aptamer.
From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift

(a) The tertiary structure of Clivia aptamer with the protruded U7 residue in red. (b) The fluorescence assay of the substitution U7 with A, G and C shows that all the substitutions are tolerated. The activated fluorescence of NBSI by Clivia mutants U7A, U7G and U7C from three independently-repeated experiments are normalized for comparison with the wild-type (WT) Clivia aptamer. Data represent the mean ± s.d. from three replicates. (c, d) Fluorescence assay of Clivia aptamer binding with NBSI in the presence of different concentration of Mg2+ (c) and varied cations (d). All the titrations are performed three times independently. Data represent the mean ± s.d. from three replicates.