Fig. 2: Neither POFUT1 nor POFUT2 is responsible for the O-fucosylation of MMRN1 EMI domains.
From: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains
a, EICs of different glycoforms of peptides containing the T216 or T265 O-fucose site from N-terminal EMI produced in HEK293T WT, POFUT1 KO, POFUT2 KO or FX KO cells. EICs of positive controls (mNOTCH1 EGF1–5 for POFUT1-mediated EGF O-fucosylaton and hTHBS1 TSR1–3 for POFUT2-mediated TSR O-fucosylation) are in Supplementary Fig. 2a,b. Spectra for the corresponding ions are in Supplementary Data 1. b, EICs of different glycoforms of peptides containing T216 or T265 O-fucose sites or N136 N-glycan site from N-terminal EMI produced in HEK293T WT cells incubated with DMSO or 6-AF. EICs of positive controls (mNOTCH1 EGF1–5 and hTHBS1 TSR1–3 for 6-AF incorporation) are in Supplementary Fig. 2c,d. Spectra for the corresponding ions are in Supplementary Data 1.
