Fig. 5: FUT10 and FUT11 are responsible for EMI O-fucosylation in HEK293T cells.
From: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains
a, EICs of different glycoforms of peptides containing the T216 or T265 O-fucose site from N-terminal EMI produced in HEK293T WT, FUT10 KO, FUT11 KO or FUT10/11 DKO cells. Red lines, O-fucose modified; black lines, unmodified. b, Relative abundances of O-fucosylated glycoforms in a were quantified. F10-11, FUT10 KO cells-clone 11; F10-16, FUT10 KO cells-clone 16; F11-23, FUT11 KO cells-clone 23; F11-43, FUT11 KO cells-clone 43; F10/11-17, FUT10/11 DKO cells-clone 17; F10/11-25, FUT10/11 DKO cells-clone 25. Statistical analysis was performed with unpaired, two-tailed t-test in Prism 7. **P < 0.01; ***P < 0.001; ****P < 0.0001 compared to control (WT cells). c, EICs of different glycoforms of peptides containing the T216 or T265 O-fucose site from N-terminal EMI produced in HEK293T WT or FUT10/11 DKO cells that co-transfected with plasmids encoding FUT10, FUT11 or EV. Red lines, O-fucose modified; black lines, unmodified. d, Quantified relative abundances of O-fucosylated glycoforms in c. F10, FUT10 plasmid; F11, FUT11 plasmid. Statistical analysis was performed with unpaired, two-tailed t-test in Prism 7. ****P < 0.0001 compared to control (EV). All data are shown as mean ± s.d. from biological triplicates of three individual transfections (Supplementary Figs. 17 and 18).
