Extended Data Fig. 5: Enrichment of RNA aptamers targeting nsp12 in UltraSelex libraries.
From: Single-step discovery of high-affinity RNA ligands by UltraSelex
a, BLI KD measurement for the reaction mixture between RNA starting pool and nsp12 proteins. b, Percentage of non-stochastic RNA species (≥2 HTS reads) in the different partitioning groups (e1–11) of UltraSelex libraries Nsp-A, Nsp-B and Nsp-C. PCR cycles were normalized. The dashed gray line represents 1% as a reference. c, Plot of the aFC values of three representative nsp12 aptamers, calculated using a top percentile value of 1.0% and a linear γ function. All details are as in Fig. 2e, but using nsp12 UltraSelex RNA aptamers. d, Sankey plot displaying the enrichment route of the top-ranked nsp12 UltraSelex aptamers. In the upper part, column AUC represents the top 0.001% of the sequences ranked by SGREELI (golden bucket), while columns e1–11 rank sequences by read abundance (golden: top 0.001%; orange: sequence observed, but ranked below 0.001%, dark green: sequences not observed in that group. The gray lines indicate the flow of the sequences between the nodes. In the magnified bottom part, the yellow bucket stands for the top 25 sequences, the gold for the top 25–top 0.001% and the orange and dark green buckets are as in the upper part.
