Extended Data Fig. 6: RNA aptamers in UltraSelex and SELEX libraries.
From: Single-step discovery of high-affinity RNA ligands by UltraSelex
a, Presence of the 47 UltraSelex top aptamers (representing the top 25 from SiR-A, SiR-B and SiR-C each) in the final round of the conventional SELEX library18. Only 5/47 sequences were found (pie chart) with ranks (by read abundance) between 3 and 484 (box plot). The box pots are defined as in Fig. 2f. b, BLI KD measurement for the reaction mixture between the RNA from SELEX round 11 pool and nsp12 proteins. The concentration of RNA pool was 1000 nM. c, The rank order distribution of the 76 high-affinity RNA aptamers in three UltraSelex libraries (all 76 species found) vs three SELEX libraries against nsp12 (9 species were absent in SELEX-A, 11 in SELEX-B and 11 species in SELEX-C, ranked by their abundance after 11 rounds of selection). The box pots are defined as in Fig. 2f.
