Fig. 4: SFPQ redox state may mediate lipoamide activity.
From: Small-molecule dissolution of stress granules by redox modulation benefits ALS models
a, Schema of the distributions of methionine (Met; 28 residues) and cysteine (Cys; 2) residues in human SFPQ; PLD, prion-like ___domain; RRM, RNA recognition motif; NOPS, NonA/paraspeckle ___domain; NLS, nuclear localization signal. b, Chemical structures of l-methionine and its nonnatural analog AHA. c, Left, representative images of HeLa cells subjected to the indicated RNAi knockdowns and cultured in complete medium (light gray) or methionine-free medium supplemented with 1 mM methionine (dark gray) or AHA (green) for 2 h, followed by 1 mM arsenate for 1 h (the experimental schematic is presented in Extended Data Fig. 7e). Stress granules were labeled with G3BP1. Right, mean ± s.d. of the percentage of stressed HeLa cells with three or more G3BP1+ SGs; n = 325–407 cells from three independent experiments. P values were calculated by a Tukey test without multiple-comparison correction. d, Schema of SFPQ as a redox sensor to modulate stress granule condensation.
