Extended Data Fig. 8: Characterization of SAMBA-CAR T cells.
From: Repurposing salicylic acid as a versatile inducer of proximity
a) Quantification of CD69 expression levels in untransduced T cells, SAMBA-CAR and WT CAR T cells after overnight co-culture with tumor cells expressing the CD19 antigen. b) Quantification of IL-2 production reversibility by washing out SA from SAMBA-CAR T cells, followed by an additional 8-hour incubation. c) ELISA measurements of IFNγ production by the indicated CAR T cells after overnight co-culture with Raji cells. d) The percentages of CD4+ to CD8+ cells after 5 days of stimulation of the indicated CAR T cells. e) FACS quantification of exhaustion markers (LAG-3 and PD-1) following 5-day stimulation of the indicated CAR T cells. f) Cytotoxic activity of the indicated CAR T cells against Raji cells at varying effector-to-target cell ratios (10:1, 5:1, or 2.5:1). WT and SAMBA-CAR T cells were co-cultured overnight with Raji cells in the absence or presence of SA, and Raji cell viability was evaluated by bioluminescence signal changes. Data represent three independent biological replicates (mean ± s.e.m.). PBMCs were collected from blood samples of three healthy blood donors.
