Extended Data Fig. 2: HIC, UV-Vis spectroscopies and binding affinities of FRTACs.
From: Polyvalent folate receptor-targeting chimeras for degradation of membrane proteins
a, Synthetic scheme for antibody-folate conjugates (FRTACs). b, 1H NMR spectrum of the key intermediate folate-PEG1000-NHS (400 MHz, DMSO). c,d, The conjugation reaction process and FAR of FR-Ctx, FR-Atz (c) and FR-Stz, FR-Ttz (d) were analyzed by HIC and UV-Vis spectroscopy. e, SPR assay analyzing of the affinity of Ctx binding to EGFR. f, SPR assay analyzing of the affinity of FAR-Atz (FAR 15) binding to FRα. g, SPR assay analyzing the affinity of FAR-Atz (FAR 15) binding to PD-L1. h, SPR assay analyzing the affinity of Atz binding to PD-L1. HIC and UV-Vis spectroscopy (c and d), SPR results (e-h) are representative of at least two independent experiments.
