Extended Data Fig. 8: Feeding of ipecac alkaloid glucosides to leaf disks expressing DGDs or S6DGDs.

Consumption of glucosides was measured because the unstable aglycone products cannot be reliably detected. a, agroinfiltration of N. benthamiana with strains harbouring constructs for overexpression of CiDGD or Ci6SDGD or empty vector (EV) respectively. Leaf disks were cut and incubated separately with indicated substrates. Note that 7-O-Me-DAIA and 7-O-Me-DAIIA are not detected in native C. ipecacuanha plants. b, agroinfiltration of N. benthamiana with strains harbouring constructs for overexpression of AsDGD1 or 2 or As6S6DGD or EV respectively. Leaf disks were cut out and incubated each with indicated substrates. AsDGDs deglycosylate all derivatives whereas AsS6DGD only shows activity towards 6-O-Me-DAIIA. 6-O-Me-DAIIA and 6-O-Me-DAIA cannot be obtained readily by Pictet-Spengler chemical reaction and were thus produced in vitro by recombinant CiDOMT1 and AsDOMT3, respectively (see methods). Bars are mean peak areas of three biological replicates relative to peak areas measured in EV, error bars are standard error of the mean SEM.