Supplementary Figure 5: SHP-1 is a common substrate for Itch and WWP2. | Nature Immunology

Supplementary Figure 5: SHP-1 is a common substrate for Itch and WWP2.

From: The E3 ligases Itch and WWP2 cooperate to limit TH2 differentiation by enhancing signaling through the TCR

Supplementary Figure 5

(a,b) Immunoblot of precipitates from HEK293T cells transfected with FLAG-SHP-1 and Itch (a) or WWP2 (b), then lysed and immunoprecipitaed with anti-FLAG antibody. (c) Immunoblot of precipitates from HEK293T cells transfected with the indicated expression vectors, lysed and immunoprecipitated with anti-FLAG antibody. (d) Immunoblot of HEK293T cells transfected with the indicated siRNA, subsequently transfected with FLAG-SHP-1 and HA-Ubiquitin expression vectors. Data are representative of two independent experiments.

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