Supplementary Figure 5: Fecal IgA and pIgR concentrations and microbiota composition in wild-type and Rorc–/– mice.
From: NIK signaling axis regulates dendritic cell function in intestinal immunity and homeostasis
a,b, ELISA analysis of IgA (a) and pIgR (b) concentrations in fecal extracts of age-matched wild-type (WT, n = 10) and Rorc–/– (n = 7) mice. c, Analysis of the commensal anaerobic bacteria Enterococcus spp. in the fecal extracts of age-matched wild-type (n = 10) and Rorc–/– (n = 7) mice by using selective agar plates. d, 16 s rRNA sequencing analysis of the genus levels of microbiota composition from age-matched wild-type (n = 5) and Rorc–/– (n = 3) mice. Each symbol (a–d) represents an individual mouse. Small horizontal lines (a–c) indicate the mean ( ± s.e.m.), and d is presented as box plots (where the middle line indicates the mean value and all individual plots are shown). Data were analyzed by unpaired two-tailed Student’s t test (a–c) or analyzed for multiple comparisons using the Holm–Sidak method (d), with P values shown above the plots. Data are representative of three independent experiments.
