Extended Data Fig. 6: Transfer of cytosolic constituents from MDSCs to CD4 T cells and NK cells.
From: Regulatory myeloid cells paralyze T cells through cell–cell transfer of the metabolite methylglyoxal
a, transfer of cytosolic constituents from MDSCs into NKT cells and CD4 T cells (n = 3). b, gating strategy used in c and d. adoptive transfer of CD45.1+CD8 T cells (106) into CD45.2+ LysM-Cre/Rosa-mito-GFP mice tumor bearing mice (B16 melanoma cells), and flow cytometric analysis for GFP fluorescence in transferred CD45.1+CD8+ cells isolated from tumor tissue and spleen directly ex vivo at 24 hours after transfer (n = 4). d, proliferation of isolated and activated CD8 T cells from tumor tissue and spleen was analyzed on day 3 using flow cytometry (c, d, representative plots of n = 4 biological independent samples).
