Extended Data Fig. 10: DMBG restores effector function of vaccination-induced CD8 T cells in cancer tissue.
From: Regulatory myeloid cells paralyze T cells through cell–cell transfer of the metabolite methylglyoxal
At d10 after s.c. B16-OVA cancer cell inoculation, mice received ovalbumin adjuvanted with CpG/alphaGalCer, anti-PD-1 (200 µg/every 3. Day) and/or DMBG in drinking water (40 mM), and analyses were performed at d17 (5 animals per group; n = 3). a, Kaplan-Meier survival curves of tumor bearing mice (n = 5). b, the expression of ovalbumin was tested in B16-melanoma in cell culture (1), ex vivo after vaccination on day 17 (2) and on day 34 after combined therapy using anti-PD-1 and DMBG alone (3,4) or in combination with vaccination (5,6) (n = 3 independen biological samples). c - e, MBo-fluorescence and glucose uptake of CD11b+ cells from spleen. f, g CD8 T cell proliferation (CFSE-dilution) in co-culture with CD11b+Ly6C+ cells or CD11b+Ly6G+ (FACSorted) from cancer tissue or spleen (numbers denote division indices). h, i, MBo-fluorescence and glucose uptake of CD8+ T cells from the spleen (a, c – e: n = 6 biological independent samples; g – i: n = 5 biological identical samples). ns = not significant; indicating no significant differences between any groups.; *p < 0.05; **p < 0.01; ***p < 0.001; two-way unpaired t-test or 2-sided Mantel-Cox test (survival curve).
