Extended Data Fig. 5: INKA1-OE mediated effects in vitro.
From: A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemness
a-c, CTRL and INKA1-OE HSPC were sorted (BFP + ) d6 post transduction and subjected to immunostaining (n = 2) and INKA1 levels were determined (a); two-sided t-test. Colocalization was quantified in (b) by Coloc2/ImageJ and Spearman’s rank correlation values for single cells with Costes’ P-value = 1.00 (**p = 0.0041); and in (c) by proximity ligation assay (PLA) with background set according to negative controls (IgG1: anti-PAK4 + rabbit IgG; IgG2 anti-INKA1 + mouse IgG); two-sided Mann-Whitney-test (b,c; ***p < 0.0001). d, Cell viability of transduced HSPC was determined via dye exclusion (PI, 7AAD; n = 9). e, CTRL and INKA1-OE LT- and ST-HSC (n = 4-5) were sorted (BFP+) d6 post transduction and subjected to cell cycle analysis by flow cytometry. f, Distribution of PAK4 protein levels in nucleus vs. cytoplasm according to confocal analysis of CTRL and INKA1-OE LT- and ST-HSC; two-sided Mann-Whitney-test (***p = 0.0005). g, Differentially expressed genes with adjusted p-value<0.01 (according to DESeq2, **p = 0.003, ***p-value range: <10-4-10-26) in either LT- or ST-HSC transduced with INKA1-OE vs CTRL (log2FC).
