Extended Data Fig. 1: INKA1 and H4K16ac are mutually exclusive.
From: A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemness
a, Representative sorting scheme to obtain LT-, ST-, IT90+- IT90- HSCs or CD34 + CD38- and CD34 + CD38 + cells from lineage depleted CB. b, Confocal images of 2 individual ST-HSC to visualize heterogeneity and representative for n = 3 independent CB pools analyzed. Scale bar = 2 μm. c, Confocal images of 4 individual, uncultured LT-HSC next to thereof derived images for INKA1 and H4K16ac channels after thresholding and binary conversion using ImageJ to visualize intracellular invers staining pattern in double dim cells of the heterogenous cell pool and representative for n = 3 independent CB. Scale bar = 4 μm. d, Percentage INKA1+ cells in HSC subsets according to INKA1+ cell gate in Fig. 1d (immunostaining, ~50 cells/population and CB, n = 3 independent CB pools). Mean ± SD. e, Confocal images and quantification (MFI) of prospectively isolated LT- and ST-HSC stained for INKA1 and CDK6 and representative for n = 3 independent CB as summarized in right panel. Gating as used for Fig. 1f is indicated. Scale bar = 2 μm.
