Extended Data Fig. 6: Selectivity of thymic myeloid cell depletion in Mgl2^DTR-eGFP mice.

(a) Experimental strategy for selective depletion of cells in Mgl2^DTR-eGFP mice. (b) Representative flow cytometric gating strategy of thymic cell populations. Cells were enriched for CD90.2 negative cells to eliminate thymocytes. Macrophages were identified by the expression of CD64. Neutrophils were identified by the expression of CD11b and Ly6g. Eosinophils were gated as CD11b⁺ SiglecF⁺. B cells were identified as CD11c⁻B220⁺ and plasmacytoid DCs (pDC) as CD11c⁺ B220⁺. Monocytes were gated as Ly6c⁺ CD11c⁻ CD11b⁺ and cDCs as MHCII⁺ CD11c⁺. (b) Quantification of cell numbers of thymic populations (gated as in a) from diphtheria toxin (DTx) treated Mgl2^WT (gray dots) or Mgl2^DTR-eGFP (green dots) (n = 9). (c) Quantification of thymic cDC numbers (gated as in Supplementary Figure 1) and thymic cDC subpopulations (gate as in Fig. 1h) from diphtheria toxin (DTx) treated Mgl2^WT (gray dots) (n = 9) or Mgl2^DTR-eGFP (green dots) (n = 9). Six to ten-week-old male and female mice were used. Small horizontal lines indicate mean, and error bars represent s.d. Data are representative of at least 3 independent experiments (b) or are pooled from at least 3 independent experiments (c, d). ns=not significant, *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA test with Tukey’s multiple comparisons test was used.