Extended Data Fig. 3: Thymic eGFP expression in Mgl2DTR−eGFP mice.
From: Type 2 cytokines in the thymus activate Sirpα+ dendritic cells to promote clonal deletion
(a) Representative flow cytometry of Mgl2-eGFP expression by CD45− EpCAM+ thymic epithelial cells (TECs). Cell were MACS enriched as CD3− CD4− CD19−TER119−. Mgl2WT mouse was used as control. (b) Representative flow cytometry of Mgl2-eGFP expression by CD11c+ cells in the thymus. The Mgl2-eGFP+ cells represent B220− XCR1− SIRPα+ cells that can be divided by the expression of MHC II and CCR7 to two main populations (as in Fig. 1h). (c) Representative flow cytometry of Mgl2-eGFP expression and CD301b protein staining of thymic SIRPα+ DCs divided according to the MHC II and CCR7 expression to DC2 (bottom) and mDC2 (top). Mgl2WT and CD0301b-PE FMO were used as control. Numbers adjacent to outlined areas represent percent cells in each. Six to ten-week-old male and female mice were used. Data are representative of at least three independent experiments.
