Extended Data Fig. 10: Severe colitis in Nlrp10D/D is associated with altered phospho NF-κB.
From: Epithelial Nlrp10 inflammasome mediates protection against intestinal autoinflammation
(a) A representative immunoblot and (b, c) quantification of phospho-NF-κB in the distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (unpaired t test **P = 0.0022; Mann-Whitney U test *P = 0.0238) (Nlrp10loxP/loxP n = 3; Nlrp10D/D n = 6). (d) A representative immunoblot (e, f) and quantification of phospho-Stat1 in distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (unpaired t test) (n = 4). (g) A representative immunoblot and (h-i) quantification of phospho-Stat3 in distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (Mann-Whitney U test) (n = 4). (j) Systemic fluorescein isothiocyanate-dextran (FITC-Dextran) levels 3 hours following FITC-Dextran gavage to Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 4 after DSS initiation (unpaired t test) (Nlrp10loxP/loxP n = 5; Nlrp10D/D n = 6). Numbers labeled in (a), (d) and (g) represent individual mice in each group. Mean ± SEM. *: p < 0.05, **: p < 0.01. Exact p values and statistical tests are presented in Supplementary Table 1.
