Fig. 5: Anti-G-CSF treatment suppresses enhanced myelopoiesis in APLAID mice.
a–f, Plcg2S707Y/+ mice (n = 4 mice per group; yellow bar) had a marked increase in the proportion and numbers of neutrophils, monocytes and their progenitors as well as their proliferation rate in the BM and spleen compared to controls (n = 2 mice per group; blue bar). g, Representative FACS plots showing cell numbers and individual frequencies of BrdU+ cells. h–o, Anti-G-CSF treatment (n = 3 mice per group; green bar) reduced myeloid counts and decreased proliferation (BrdU+ cells) in the BM and spleen relative to isotype control-treated APLAID mutant mice (n = 3 mice per group; yellow bar), and to a comparable level as that seen in Plcg2+/+ mice (n = 1 mouse, blue bar). p, Representative FACS plots showing individual cell numbers and frequencies of BrdU+ cells under anti-G-CSF treatment or isotype control. Error bars represent the mean ± s.e.m. All mice were 4–5 weeks of age. Cell numbers between two groups were determined by an unpaired two-sided Student’s t-test.
