Extended Data Fig. 1: WT BMMCs express several integrin receptors and form β1 integrin-containing adhesion structures on FN-coated 2D surfaces.
From: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
(a) BMMCs that were generated in the presence of IL-3, KITLG and IL-4 over 6 weeks were analyzed by flow cytometry for the cell surface expression of α and β integrin subunits. Isotype control stainings are displayed in blue, integrin subunit-specific stainings in red. (b) Interaction of BMMCs with FN-coated glass slides 30 min after IgE/DNP–HSA-induced cell spreading. Confocal fluorescence microscopy of BMMCs stained for F-actin and the focal adhesion components integrin α5, paxillin (PXN), pan-talin (TLN), vinculin (VCL) and active integrin β1 (9EG7). Representative images of n = 10 cells from one of two independent experiments. Scale bars: 5 µm (b left), 2 µm (b right).
