Extended Data Fig. 1: Gating strategy and population dynamics of myeloid cells in the murine mammary gland during lactation.

a-c, Representative flow cytometry plots for the gating strategy of eosinophils, neutrophils, Ly6C^hi monocytes, F4/80^lo macrophages (Mac), F4/80^hi macrophages, liMacs and dendritic cells (DC) (pre-gated on live, singlets and CD45⁺ cells) (a), violin plots of total cell counts (b) and bar graph with the percentages of total myeloid cells (c) in mammary glands from virgin, lactating (day 10 pp) and post-lactating (day 21 pp) wild-type mice. Data (n = 5-6 per timepoint) are pooled from 6 independent experiments. Kruskal-Wallis test was corrected with Dunn’s multiple comparisons test, *p < 0.05; **p < 0.01; ***p < 0.001. d, UMAP plot (pre-gated on CD45⁺CD11b⁺ and/or CD11c⁺) showing the myeloid compartment of the mammary glands in late gestation (at E18.5), analyzed by flow cytometry. Heatmap shows the mean marker expression. Data were transformed and percentile normalized. Related to Fig. 1.