Extended Data Fig. 5: tDC convert into DC2.
a tDC were purified using the gating strategy described in Fig. 1f. Top graph shows cell purity after sort. Bottom graphs show the outcome of transferred cells in the spleen of recipient mice analyzed at 4-days. One representative of 6 experiments. b Bar graphs (mean + SD) of the total number of splenic DC in hCD2-iCre+/− Cx3cr1DTR+/− mice inoculated with DT and analyzed 1 day after (n = 5 mice in 4 experiments), or inoculated with DT every second day and analyzed 7 days later (n = 5 mice in 4 experiments). ‘C’ represents control mice (n = 8 mice in 4 experiments), which are a combination of hCD2-iCre−/− Cx3cr1DTR+/− mice inoculated with DT or hCD2-iCre+/− Cx3cr1DTR+/− mice inoculated with PBS (no differences were observed between these control mice). Statistics determined by one-way ANOVA with Tukey’s multiple comparison test. c As in (b), but BM progenitors from hCD2EYFP CX3CR1DTR mice were analyzed after 7 days of DT inoculation (n = 4 mice). Control mice are hCD2EYFP CX3CR1DTR mice inoculated with PBS (n = 3 mice). Bar graphs (mean + SD) of the frequency of total progenitor populations from Fig. 3j. Data pooled from 2 experiments. Statistical differences were determined by unpaired two-tailed t-test. d Mouse splenic DC subsets were sorted using the gating strategy show in Figs. 1f and 4g, and analyzed by PCR assay for IgH D-J rearrangements. Actin and IgH germline (GL) are also shown. One representative of 3 experiments. e DC2 from CD300cTdT mice were gated as indicated in Fig. 4f, and further separated in TdTomato+ and TdTomato-. Heatmap indicating the relative expression (Z-score of gMFI) of surface markers. Z-score was calculated base of n = 2 mice in 2 experiments.
