Fig. 5: scRNA-seq analysis of NF-κB target genes in activated B cells.

a–d, scRNA-seq was carried out with anti-IgM-activated B cells from RelA^fl/fl (control) and RelA^fl/flxCd19-cre (RelA cKO) for 0, 1 and 4 h or from C57BL/6 (WT) and Rel^−/− B cells treated with anti-IgM for 0, 1 and 18 h. In total, 10,000 B cells were assayed from each condition. Data analysis was carried out by Seurat as described in Methods. Dotplot visualizations of differential gene expression patterns of select NF-κB target genes are shown. Gene expression changes are plotted by both scaled average expression (avg. exp., illustrated by dot color) and the percentage of cells found to express that gene (exp (%), depicted by the size of the dot). Cellular origin of libraries is indicated on the y axis. a, Single-cell analysis of RelA-selective genes from Fig. 4a (x axis). Expression levels and proportions of gene-expressing cells in control and RelA cKO B cells are depicted at 0, 1 and 4 h. b, Expression patterns of RelA-selective genes from Fig. 4a are depicted in WT and Rel-deficient B cells activated for 0, 1 and 18 h. c, Single-cell expression patterns of additional Rel-repressed genes (from cluster R2 in Fig. 3f) in WT and Rel-deficient B cells activated for 0, 1 and 18 h. d, Single-cell analysis of Rel target genes (from clusters R3 and R4 in Fig. 3f) in WT and Rel-deficient B cells activated for 0, 1 and 18 h. Additional quantitation for all patterns is shown in Supplementary Fig. 6c–f. An asterisk indicates that A93002 = A930024E05Rik.