Extended Data Fig. 5: CXCR6 deletion increases Aβ plaques in 5xFAD mice.
From: CXCR6 orchestrates brain CD8+ T cell residency and limits mouse Alzheimer’s disease pathology
a, Representative immunofluorescence images (upper) and quantification of number (lower left) and burden (% of area) (lower right) of Aβ plaques in 10-month-old female 5xFAD;Cxcr6+/+ (n = 6 sections for plaque number; n = 4 sections for plaque burden) and 5xFAD;Cxcr6–/– (n = 8 sections for plaque number; n = 16 sections for plaque burden) mice. Scale bar, 250 μm. b, Representative Aβ immunostaining in composite wide-field brain sections from indicated 10-month-old female mice. c, Immunofluorescence analysis of number and burden of Aβ plaques in 4-month-old male 5xFAD;Cxcr6+/+ (n = 10 sections for plaque number; n = 8 sections for plaque burden) and 5xFAD;Cxcr6–/– (n = 12 sections for plaque number; n = 8 sections for plaque burden) mice. Scale bar, 250 μm. d, Immunofluorescence analysis of number and burden of Aβ plaques in 10-month-old male 5xFAD;Cxcr6+/+ (n = 6 sections for plaque number; n = 6 sections for plaque burden) and 5xFAD;Cxcr6–/– (n = 6 sections for plaque number; n = 6 sections for plaque burden). Scale bar, 250 μm. e, f, Soluble and insoluble fractions of Aβ1-40 and Aβ1-42 protein levels in the hippocampus or cortex of 4-month-old female (e) and male (f) 5xFAD;Cxcr6+/+ (n = 4 for female and 4 for male) and 5xFAD;Cxcr6–/– (n = 4 for female and 4 for male) mice. g, Total arm entries from spontaneous Y maze alternation testing of 5xFAD;Cxcr6+/+ (n = 10 for female and 9 for male) and 5xFAD;Cxcr6–/– (n = 9 for female and 10 for male) mice. h, Filamentous, compact, and inert Aβ plaque phenotypes in brain sections from 10-month-old female 5xFAD;Cxcr6+/+ (n = 6 sections) and 5xFAD;Cxcr6–/– (n = 8 sections) mice. Data were analyzed by two-tailed unpaired Student’s t-test (a, c, d for plaque burden and e–h) or two-tailed unpaired t-test with Welch’s correction for plaque number (a, c, d). Data are shown as mean ± s.e.m. in a and c–h; NS, not significant. Data were pooled from at least two (a, c, d, g) or one (b, e, f, h) independent experiments.
