Fig. 6: Age-related changes in probability of generation of A2/M158+CD8+ TCRs.
a, Frequency of A2/M158+CD8+ TCRβ motifs in bulk repertoires of HLA-A*2-expressing and negative donors and b, across decades of human life for HLA-A*2+ donors. Each dot is the cumulative frequency of TCRβ chains from A2/M158+CD8+ T cells in bulk TCRβ repertoires. Dots represent individual donors, HLA-A*2+ age group 0–9, n = 6; 10–19, n = 14; 20–39, n = 157; 40–59, n = 111; 60–79, n = 24; 80–103, n = 17 and HLA-A*2− age group age group 0–9, n = 22; 10–19, n = 24; 20–39, n = 189; 40–59, n = 150; 60–79, n = 29; 80–103, n = 9. c,d, Probabilities of generation (Pgen; log10 transformed) for all single TCRα (c) and TCRβ (d) chains proteins from newborns, children, adults and older adults estimated with TCRdist. e,f, Number of nucleotide insertions (e) and deletions (f) for all single TCRβ chains. g, Pgen (log10 transformed) for TCR β-chain proteins that include TRBV19 (left) or other V (right) gene segments in newborns, children, adults and older adults generated with TCRdist. Box plots represent the median (middle bar), 75% quartile (upper hinge) and 25% quartile (lower hinge) with whiskers extending 1.5 × interquartile range, dots represent individual clonotypes derived from n = 6 newborns, n = 12 children, n = 8 adults and n = 10 older adults, with clone size indicated by symbol size. Statistical analysis of Pgen and for the number of insertions and deletions between age groups utilized a two-sided mixed-effects model with donor encoded as a random effect, as described in Methods. P values were adjusted (Padj) for multiple testing with the Benjamini–Hochberg false discovery rate (FDR) method. N, newborn; C, children; A, adult; OA, older adult.
