Extended Data Fig. 9: Protein level expression of interferon-stimulated gene (Mx1) and IFN-γ in lung cells, and CD4+ T cells, respectively. | Nature Immunology

Extended Data Fig. 9: Protein level expression of interferon-stimulated gene (Mx1) and IFN-γ in lung cells, and CD4+ T cells, respectively.

From: BCG vaccination stimulates integrated organ immunity by feedback of the adaptive immune response to imprint prolonged innate antiviral resistance

Extended Data Fig. 9

a, Mx1 median fluorescent intensity (MFI) of lung myeloid and alveolar type II epithelial cells detected using Mx1-GFP reporter mice at various timepoints post-vaccination with BCG intravenously (n = 3-5). Data representative of two independent experiments in lung myeloid cells and one representative experiment in alveolar type II cells; n = 5 for day 21, n = 4 for all other timepoints. b, Frequency of IFN-g+ cells (% of cell population of interest) in vivo, as measured by flow cytometry staining 6 h following Brefeldin A injection in mice. Data from one representative experiment; n = 7 for BCG, n = 4 for Unimmunized. Statistical analysis was performed by Two-way ANOVA with Dunnet’s test (a; myeloid cells) Sidak’s multiple comparisons test (b), and with two-tailed Mann-Whitney test (a; alveolar type II cells). *, P < 0.01; ****, P < 0.0001.

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