Extended Data Fig. 7: ST2⁺ and CXCR3⁺ Treg cells interact with distinct cell types.

a, FACS histogram overlays showing expression of GzmB, CTLA-4, Areg, CD25, IL-10 and CD39 in ST2⁺ and CXCR3⁺ VAT Treg cells. b, Graph showing frequency of IL-10-GFP expressing ST2⁺ VAT Treg cells from male (n = 16) and female (n = 9) mice. c, Dot plot shows reciprocal interaction between ST2⁺ and CXCR3⁺ VAT Treg cells and the VAT microenvironment. d, FACS gating scheme used to identify different myeloid cells in the VAT. e, Graph shows Ccl2-RFP mean fluorescence index (MFI) in VAT dendritic cells, macrophage subsets and neutrophils from male (n = 8) and female (n = 10) mice. f, FACS plots show frequency of Ccl2-RFP⁺ cells in male and female VAT resident and inflammatory macrophages. Quantification on the right. Symbols represent individual mice; horizontal lines indicate means. Data are pooled from at least two independent experiments (b, e, f); FACS plots are representative (a, d, f). Error bars indicate the standard deviation. Statistical analyses were performed using two-way ANOVA (e, f) or unpaired, two-tailed Student’s t-test (b).