Extended Data Fig. 9: Altered VAT transcriptional profiles after Treg-cell-specific deletion of T-bet and PPARγ.

a-e, Volcano plots shows genes differentially expressed between total VAT of female vs male control mice (a), female vs male Pparg^fl/flFoxp3^Cre (b), female vs male Tbx21^fl/flFoxp3^Cre (c), female Tbx21^fl/flFoxp3^Cre vs control (d) and male Tbx21^fl/flFoxp3^Cre vs control (e) mice. f, KEGG (top) and GO (bottom) pathway enrichment analysis for differentially expressed genes in female VAT of Tbx21^fl/flFoxp3^Cre vs control mice. Positive values refer to pathways upregulated (red), negative values refer to pathways downregulated (blue) in the VAT. g, Flow cytometry plots showing glucose uptake measured by 2-NBDG staining in ST2⁺ and CXCR3⁺ male and female VAT Treg cells. Quantification on the right (n = 3 females, n = 6 males). h, i, Volcano plots showing genes differentially expressed between total VAT of female Pparg^fl/flFoxp3^Cre vs control (i) and male Pparg^fl/flFoxp3^Cre vs control (j) mice. Each dot in volcano plot represents a gene; genes highlighted in red are up-regulated and genes highlighted in blue are down-regulated.