Extended Data Fig. 1: Generation of preclinical mouse models expressing the heavy chains of NGS-04 and 10E8UCA, related to Fig. 1.

a, Representative flow cytometry of bone marrow progenitors isolated from 8–10‐week‐old wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice separated by Hardy classification into: early (B220⁺CD43⁺) and late (B220⁺CD43⁻) B cells (top panel); early (A–C) B cell subfractions (middle panel); and the late (D–F) subfractions in the bone marrow (bottom panel). Data is representative of two independent experiments (n = 3–4 mice/independent group). b, Quantification of flow cytometry of bone marrow progenitors, as in a. Error bars indicate mean ± SD from mice in each group. Data are pooled from two independent experiments (n = 3–4 per treatment) for wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice; MPER-HuGL18^H data presented for comparative purposes (n = 4 mice from one experiment). c, Representative flow cytometry of CD4⁺/CD8⁺ T cells and T2/MZB (marginal zone B cells) cells isolated from spleen samples of 8–10‐week‐old wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice, as in Fig. 1a,b. d, Quantification of T and B cell subfractions, as in c. Error bars indicate mean ± SD from mice in each group. Data are pooled from two independent experiments (n = 3–4 per treatment) for wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice, with some values repeated from Fig. 1a, b for comparison; MPER-HuGL18^H data also presented for comparative purposes (n = 4 mice from one experiment). e, Representative flow cytometry histograms showing expression of B220, IgM and IgD in follicular B cells in spleen (green, WT; red, 10E8-NGS-04; yellow, 10E8-UCA and blue, MPER-HuGL18). Data representative of two independent experiments (n = 3–4 mice per treatment) for wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice; MPER-HuGL18^H data presented for comparative purposes (n = 4 mice from one experiment).