Fig. 6: Replenishment of molecularly distinct MkPs by Vwf-tdTomato^– multi-HSCs and Vwf-tdTomato⁺ P-HSCs.

a, DEGs (adjusted P < 0.05, combined Wilcoxon/Fisher’s exact test, absolute log₂(fold change) > 0.5; blue, genes overlapping with the DEGs comparing total P-MkPs and total multi-MkPs in Fig. 4a; red, genes not overlapping with the DEGs in Fig. 4a) when comparing CD48⁺ (left; n = 122 cells) or CD48^– (right; n = 51 cells) molecularly defined MkPs replenished by single Vwf-tdTomato⁻ multi-HSCs (multi-MkPs) to CD48^– molecularly defined MkPs replenished by single Vwf-tdTomato⁺ P-HSCs (P-MkPs; n = 101 cells). Cd48, Vwf, Cd24a and Itga2 are highlighted in blue. b, Distribution of Vwf log₂(mRNA expression) and percentage of Vwf transcript-positive cells (left) and Vwf-tdTomato reporter fluorescence distribution (right) in CD48^– and CD48⁺ molecularly defined multi-MkPs and CD48^– P-MkPs. The numbers of analyzed single cells are indicated below the violin plots. c, DEGs (adjusted P < 0.05, combined Wilcoxon/Fisher’s exact test, absolute log₂(fold change) > 0.5; blue, genes overlapping with the DEGs comparing total P-MkPs and total multi-MkPs in Fig. 4a; red, genes not overlapping with the DEGs in Fig. 4a) when comparing Cd48 transcript-positive (left) or Cd48 transcript-negative (right) multi-MkPs (n = 102 and 71 cells, respectively) to Cd48 transcript-negative P-MkPs (n = 114 cells). Cd48, Vwf, Cd24a and Itga2 are highlighted in blue. d, DEGs (adjusted P < 0.05, combined Wilcoxon/Fisher’s exact test, absolute log₂(fold change) > 0.5; blue, genes overlapping with the DEGs comparing total P-MkPs and total multi-MkPs in Fig. 4a; red, genes not overlapping with the DEGs in Fig. 4a) when comparing Cd48 transcript-positive (n = 102 cells) to Cd48 transcript-negative (n = 71 cells) multi-MkPs. A detailed list of detected DEGs is provided in Supplementary Table 4.