Extended Data Fig. 4: Quantification of non-epithelial stromal cells subsets and aaTEC gating. | Nature Immunology

Extended Data Fig. 4: Quantification of non-epithelial stromal cells subsets and aaTEC gating.

From: Age-related epithelial defects limit thymic function and regeneration

Extended Data Fig. 4

a–c, Concatenated flow cytometry plots and quantities for cell populations within the fibroblast (a), endothelial (b), and “other” (c) cell lineages in 2-mo (n = 10) and 18-mo (n = 10) mice. c, Concatenated flow cytometry plots and quantities for pericytes (PC), vascular smooth muscle cells (vSMC) and mesothelial cells (MEC) (n = 10/age). d, Frequency and numbers of DN-TEC across lifespan: 2-mo (n = 14), 6-mo (n = 5), 9mo (n = 15), 12-mo (n = 5), and 18+mo (n = 18). e, Violin plots with extensive list of aaTEC1 and aaTEC2 markers. f, Gating strategy for aaTECs. aaTEC1 were first gated on CD45TER119 then PDGFRα-CD31 cells. EpCAM+MHCII+ cells were gated as the whole TEC compartment, then mTECs and cTECs were excluded by taking the UEA1Ly51 double negative fraction and gating on CLDN3. aaTEC2 were also first gated on CD45TER119 then PDGFRα-CD31 cells. EpCAMMHCII+ cells were then gated and PDPN+PDGFRβ- were classed at aaTEC2. Summary data represents mean ± SEM; each dot represents an individual biological replicate. Statistics for a–c were generated using two-tailed Mann–Whitney tests comparing within individual populations and for d using the Kruskal–Wallis test with Dunns correction.

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