Extended Data Fig. 8: scRNA-seq analysis of WT and Irf8 + 32^H/H BM and spleen.

(a) Clustering of BM lin⁻ Flt3⁺ Kit^int-lo cells from WT and H/H mice projected onto a UMAP space (22,839 total cells, with 11,441 from WT and 11,398 from H/H mice). (b) Distributions of WT (blue) and H/H (red) cells across the UMAP in (a). (c) Dot plot of top 10 differentially expressed genes for each cluster in (a). (d) Clustering of BM lin⁻ Flt3⁺ Kit^int-lo cells in (a) split by individual biological replicates. (e) Normalized and scaled Irf8 expression in WT (upper panel) and H/H (lower panel) cells. (f) Violin plot of normalized and scaled Irf8 expression across clusters. WT (blue) and H/H (red) cells are displayed side by side for each cluster. Statistical significance was determined by a two-tailed Wilcoxon rank sum test, applying a log₂ fold-change threshold of 0.1. P values are indicated above the graphs for statistically significant comparisons. (g) Clustering of splenic CD11c⁺ cells from Fig. 5a split by individual biological replicates. (h) Normalized and scaled expressions of selected cluster marker genes in splenic CD11c⁺ cells.