Extended Data Fig. 1: The Irf8 + 32 kb comprises four AICE motifs.
From: Optimization of the Irf8 +32-kb enhancer disrupts dendritic cell lineage segregation
(a) ATAC-seq and ChIP-seq tracks for the indicated cell types are shown for the Irf8 locus. The locations of the Irf8 + 32 kb, +41 kb and +56 kb enhancers are highlighted. The tracks are displayed using the UCSC genome browser with vertical auto scale. (b) A zoomed-in view of a 1,168 bp region surrounding the Irf8 + 32 kb enhancer highlighting the locations of AICEs. The previously deleted 149 bp (blue) and 421 bp (red) regions from Irf8 + 32 5′−/− mice and Irf8 + 32−/− mice, respectively23, are indicated relative to the AICEs. (c) Sequence of the 421 bp region deleted previously in Irf8 + 32−/− mice23, with AICEs underlined and highlighted in blue. The AICEs are displayed from left to right in the order of site 1, site 4, site 3, and site 2.
