Supplementary Figure 5: Cell viability test of the AAV–Cpf1 system.
From: One-step generation of modular CAR-T cells with AAV–Cpf1
a, Estimation of cells lost because of electroporation, or because of the combined effects of electroporation and AAV infection for 24 h (cell culture replicates, n = 3). b, Live cell percentage before and after electroporation along a time course, in which viability was determined by trypan blue staining. Cell viability showed no significant difference before and after electroporation across all time points. Kruskal–Wallis test was used to assess statistical significance (P = 0.0587) (cell culture replicates, n = 3). Data are shown as mean ± s.e.m. c, Engineered CD22BBz KIKO CAR-T cell growth time course, with T cells stimulated with mitomycin-C-pretreated NALM6 cells at a 1:1 ratio each week. The total number of cells is shown along different time points for a maximum of 26 d. Arrows indicate stimulation time points (cell culture replicates, n = 3). Data are shown as mean ± s.e.m.
