Supplementary Figure 8: Additional comparisons of crRNA array abundances.
From: In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens
a–c, Scatter plot of MCAP-MET crRNA array abundance in cell pools versus primary tumors (a), cell pools versus lung metastases (b) and primary tumors versus lung metastases (c). Data are shown in terms of average log2 RPM across the indicated sample type. To illustrate the null distribution, the linear regression line of NTC-NTC control arrays is overlain. Shading on the regression line denotes the 95% confidence interval. d, Scatter plot of MCAP-MET single knockout (SKO; n = 26 genes) and double knockout (DKO; n = 325 gene pairs) abundances in cell pools (n = 6 cell replicates) versus lung metastases (n = 37 from ten mice). Data shown in terms of average log2 RPM for the indicated sample type, after first averaging the constituent crRNA arrays for each gene/gene pair. The linear regression was calculated over the entire library, with the 95% confidence interval shaded in. Significant outliers (two-sided outlier test, adjusted P < 0.05) are outlined and enlarged, with s.e.m. error bars.
