Extended Data Fig. 3: Fluorescence and smFRET data for donor and acceptor labeled Sf-mGluR2 immobilized under fixed cell conditions.
From: Single-molecule FRET imaging of GPCR dimers in living cells
a, Representative fluorescence (top)- and FRET (bottom) time traces for individual receptors. b, Distributions of the total intensity (donor + acceptor) and acceptor intensity during smFRET. The distributions were fit to a single gaussian function, yielding a mean total intensity of 458 photons/frame (full width at half maximum (FWHM) of 218) and a mean acceptor intensity of 279 photons/frame (FWHM of 288). c, Lifetime of smFRET events for Sf-mGluR2 in fixed cells. The lifetime distribution was fit to a single exponential to produce the decay constant τ. The data in panels b and c are derived from 124 molecules and a total of 16 cells.
