Extended Data Fig. 5: RNA kinetic parameter comparison by cell volume and cell-cycle stages.

a-b, Violin plots showing the distribution of RNA concentration (a), synthesis, degradation, export and cytoplasmic translocation (b) for all detected genes averaged across single cells. n = 2792 cells for large cells and 2669 cells for small cells in a-b. c, violin plots showing the distribution of cell volumes (in voxels) in different cell-cycle stages. ANOVA test. n = 881, 2324, 2256 cells in G1, G1/S and G2/M, respectively. d, synthesis, degradation, export, and cytoplasmic translocation for all detected genes averaged across single cells in each cell cycle phase and separated by cell volumes. Units of α is RNA concentration/hr, where RNA concentration is defined by copies/voxel (voxel = 200 nm x 200 nm x 350 nm). Units of β, λ, γ are hr⁻¹. Boxplots indicate mean and 25-75% quantile. n = 239, 1484, 1069 cells for G1, G1/S and G2/M of large cells, respectively, and 642, 840, 1187 cells for G1, G1/S and G2/M of small cells. Paired t-test. ***p < 0.001. *p < 0.05. e-h, Examples of pairwise correlation in Fig. 3d, showing scatter plots of the relationships between G1 and G2/M. Pearson’s correlation coefficients from left to right: α (ρ = 0.99), β (ρ = 0.60), λ (ρ = 0.46), γ (ρ = 0.04).