Extended Data Fig. 1: MISAR-seq: workflow, design and library distribution. | Nature Methods

Extended Data Fig. 1: MISAR-seq: workflow, design and library distribution.

From: Simultaneous profiling of spatial gene expression and chromatin accessibility during mouse brain development

Extended Data Fig. 1

a, MISAR-seq workflow. The tissue section was first subjected to Tn5 tagmentation, followed by reverse transcription (RT) and finally two rounds of barcode (50 kinds each) ligation on chip. R1, Read1 adaptor. R2, Read2 adaptor. b, The composition of microfluidic devices used in this study, including PDMS slab, barcode inlets, acrylic clamp and suction hole. c, AutoCAD design of PDMS chip with 50 μm channel width. d, Verification of leakage or diffusion by Cy3 and Fam dye. e-g, Size distributions of ATAC library (e), cDNA amplicons (f) and cDNA library (g).

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