Extended Data Fig. 2: ASAP4.0 to ASAP4.2.
From: A positively tuned voltage indicator for extended electrical recordings in the brain
(A) Steady-state responses from command voltage patch clamping in HEK 293 A cells for ASAP4.0 to ASAP4.2. Responses are normalized to –70 mV. The test pulses before each step are 2-ms square steps from –70 to +30 mV (B) Left, ∆F/F0 over voltage steps for the parent construct ASAP2f L146G S147T R414Q, as well as ASAP3 and the first three ASAP4 variants. Error bars are SEM. Right, brightness vs. voltage plotted as a fraction of the maximum brightness for each indicator. Data are displayed as mean ± SEM. The gray box highlights the physiological range from –70 to +30 mV. (C) Screening all combinations of F148X N149X in ASAP4.1 (ASAP4.0 T206H) yielded F148P N149V, creating ASAP4.2.
