Extended Data Fig. 4: Photobleaching of ASAP indicators under one-photon and two-photon excitation. | Nature Methods

Extended Data Fig. 4: Photobleaching of ASAP indicators under one-photon and two-photon excitation.

From: A positively tuned voltage indicator for extended electrical recordings in the brain

Extended Data Fig. 4

(A) One-photon photobleaching curves over 5 min of continuous illumination in cultured HEK293-Kir2.1 cells, using blue light with a peak wavelength at 453 nm, tested with varying intensities. Shaded areas represent SEM. (B) Resonant-scanning two-photon photobleaching in HEK293-Kir2.1 cells at 518 and 888 mW/mm2). Shaded areas represent SEM. (C) Galvanometric-scanning two-photon photobleaching in HEK293-Kir2.1 cells at 960 mW/mm2. After 5 min, cells were incubated the dark and imaged at single time points 2.7, 3.3, and 5.0 min later. Shaded areas represent SEM.

Back to article page