Extended Data Fig. 6: High-speed visualization of chromosome targeting of FP-tagged condensin I in genome-edited HCT116 cells.
From: StayGold variants for molecular fusion and membrane-targeting applications
a, After release from cell cycle arrest, genome-edited HCT116 cells (#897) were imaged under 488 nm excitation for observation of CAP-H-td5oxStayGold and under 637 nm excitation for observation of SiR-DNA–labeled chromosomes by spinning-disk LSCM (SpinSR10) at 1 frame per second. Merged images at the indicated times (min:s). Scale bars, 10 µm. See Supplementary Video 3. b, Photostability comparison between CAP-H-td5oxStayGold and CAP-H-mClover3 under the same optical conditions. Illumination intensity, 2.24 W cm−2. Genome-edited HCT116 cells (#897, top vs. #899, bottom) during prometaphase were volume imaged (z-step, 0.25 µm; z-range, 2.5 µm) every 6.9 s over a total period of 278 s, with continuous excitation at 488 nm. Each gray scale indicates that the lowest and highest fluorescence intensities. Scale bar, 10 µm. See Supplementary Video 4.
